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Figure 7


Fig. 7. Localization of Lasp-1 and 140 kDa palladin in astrocytes. The 140 kDa isoform of palladin was detected with the polyclonal 4IgNT antibody characterized in Fig. 2, and Lasp-1 was detected with the 3H8 monoclonal antibody. In the merged images, palladin staining is shown in green and Lasp-1 staining is shown in red. Both Lasp-1 and the 140 kDa isoform of palladin colocalize in stress fibers of astrocytes (A-F), although Lasp-1 exhibits a more pronounced localization to focal adhesions. In longer-term cultures of astrocytes that have formed monolayers of epithelial-like cells, both palladin and Lasp-1 are detected within cell-cell-contacts (G-I). Bars, 50 µm.





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