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Figure 2


Fig. 2. Accumulation of LAMP1-positive vacuoles is specific to suppression of hVps34 expression. (A) U-251 cells infected with retrovirus harboring the control or two different hVps34-specific RNAi sequences (KD and KD2) were subjected to immunoblot analysis as described previously. (B) Control, hVps34 KD and hVps34 KD2 cells were seeded at 100,000 cells/dish in 35 mm dishes. 48 hours later, cells were examined by immunofluorescence microscopy using an antibody against LAMP1. Bar, 10 µm.





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