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Figure 7


Fig. 7. LAMP1-positive vacuoles formed in hVps34 KD cells are depleted of PtdIns(3)P, as measured by localization of a GFP-2xFYVE probe. Control or hVps34 KD cells were transfected with a vector encoding GFP-2xFYVE. After 16 hours, the cells were processed for immunofluorescence, using primary antibodies against GFP and LAMP1. Bar, 10 µm.





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