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Figure 4


Fig. 4. PTP1B puncta co-localize with FAK in fixed and living cells. (A-C) PTP1B null cells stably expressing PTP1B DA double labeled with anti-FAK and anti-PTP1B, followed by secondary antibodies conjugated with Alexa Fluor 568 (A) and Alexa Fluor 488 (B), respectively. Note the high overlap of both proteins at the cell margins (arrows). (D) PTP1B null cells co-transfected with mRFP-PTP1B DA and GFP-FAK analyzed by time-lapse confocal microscopy after 24 hours. Note that most PTP1B DA puncta also contain FAK (in yellow), however, in instances of non co-localization, FAK has an elongated distribution. Arrows indicate puncta containing both PTP1B DA and FAK that move or disappear from the plane of focus without changing their relative fluorescence. Arrowheads indicate puncta that fuse or split. Bars, 20 µm.





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