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Figure 9


Fig. 9. Lamellar extensions in cells lacking PTP1B are unstable and contain immature cell-matrix sites. PTP1B null cells (null) and cells reconstituted with wild-type PTP1B (+wt) were transfected with GFP-vinculin or GFP-paxillin and analyzed by time-lapse confocal microscopy. Note that PTP1B null cells produce lamellar extensions and assemble vinculin and paxillin foci (arrows); however, the lamellae quickly retract (arrowheads). By contrast, cells expressing wild-type PTP1B extend lamellae and assemble vinculin and paxillin foci that are persistent in time (arrows). Bar: 30 µm. The percentage of paxillin foci that also contain zyxin in PTP1B null cells and cells stably expressing wild-type PTP1B is shown in the histogram in the bottom, right panel. Results shown are the mean ± s.d. of three independent experiments (n=44 PTP1B null cells; n=39 cells expressing wild-type PTP1B). The number of cell-matrix sites per cell ranged from 50-100.





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