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Figure 2


Fig. 2. AtPIN immuno-fluorescence staining in plant tissues. (A-C) Arabidopsis root expressing AtPIN1::GFP. (A) Staining pattern with AtPIN-Cy3 (red). (B) AtPIN1::GFP expression pattern (green). (C) Merged picture showing that AtPIN colocalises with AtPIN1::GFP (yellow) and stains additional cell lines (red). Nuclear staining with Hoechst 33342 (blue). (D) pin-formed1 mutant naked stem with AtPIN-Cy3. A PIN-like polar pattern is still observed (arrow); x indicates autofluorescence of xylem elements. (E) Longitudinal section of Zea mays root labelled with AtPIN-FITC with the typical ladder-pattern. (F-G) Longitudinal sections of BFA-treated Zea mays roots stained with AtPIN-FITC. (F) 90 minutes BFA, (G) 150 minutes BFA. Occurrence of `BFA compartments' and progressive disappearance of the polar PIN pattern. Bars 20 µm.





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