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Fig. 1. Antimycin A and chloramphenicol induce triglyceride accumulation in 3T3-L1 preadipocytes. (A) Photomicrographs of 3T3-L1 cells (a) not treated or treated (b) for 8 days with 10 nM AA, (c) for 16 days with 100 µg/ml chloramphenicol and (d) for 8 days with an adipogenic cocktail. Cells were stained for the presence of neutral lipids with Oil Red O. (B-C) Quantitative determination of triglyceride accumulation in 3T3-L1 cells (B) incubated without a drug (controls, CTL) or incubated with the indicated concentrations for 8 days with antimycin A and (C) for 16 days with chloramphenicol. The absorbance of cell monolayers was spectrophotometrically determined at 490 nm after Oil Red O staining. Results are expressed in optical density (O.D.) as the mean ± s.d. for n=3 experiments. **P<0.01 and ***P<0.001, significantly different from control cells. Magnification, 150x.