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Figure 5


Fig. 5. (A) Comparison of the results obtained with RT-PCR and DNA microarray analyses for FAS, Clic4 and CPT-2 genes. Cells were incubated for the indicated times with the adipogenic cocktail (DIFF) or 10 nM antimycin A (AA) before RNA extraction, reversetranscription and amplification in the presence of SYBR Green and specific primers. TBP was used as a house keeping gene for data normalization. (B) Western blot analysis for GPDmit performed on 20 µg of clear cell lysate proteins prepared from 3T3-L1 cells incubated or not (controls, CTL) for 2, 4, 6 or 8 days with 10 nM AA. Equal protein loading was controlled by the immunodetection of PARP.





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