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Figure 10


Fig. 10. Effects of recombinant Wnt3a on the survival and activity of mature osteoclasts. Osteoclasts were generated from peripheral blood mononuclear cells cultured in the presence of RANKL and M-CSF for 14 days, and treated with recombinant Wnt3a (50 ng/ml) or vehicle alone for the final 48 hours of culture. The number of TRAP-positive osteoclasts and the area of bone resorption were quantified. Wnt3a had no reproducible effect on osteoclast survival or their resorptive activity. Data are mean ± s.e.m., n=5.





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