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Figure 2


Fig. 2. Nuclear translocation of ß-catenin in osteoblasts. (A) Wnt3a-conditioned medium (Wnt3a) stimulated ß-catenin translocation in MC3T3-E1 cells (arrows) compared with cells incubated with control conditioned medium (L-cells). Bar, 5 µm. (B) Dose-dependence of Wnt3a induced ß-catenin translocation in MC3T3-E1 cells. No effect was observed in cells exposed to L-cell conditioned medium at all concentrations tested. (C) Dkk-1 inhibited the nuclear accumulation of ß-catenin induced by 25% Wnt3a in MC3T3-E1 cells (mean ± s.e.m., n=12; **P<0.01, ***P<0.001; n.s., not significant).





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