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Figure 6


Fig. 6. Wnt signalling inhibits osteoclastogenesis in mouse primary osteoblast/mononuclear spleen cell co-cultures. (A) Inhibition of GSK3ß with LiCl (5-20 mM) inhibited the formation of TRAP-positive multinucleated cells compared with cells incubated with NaCl (5-20 mM) over 12 days in culture. (B) LiCl inhibited mononuclear cell fusion (arrows indicate characteristic cell fusion in control cultures; open circles, mononuclear cells; dashed arrow, rare fused cell in LiCl-treated cultures). TRAP stained osteoclasts predominate in NaCl-treated cultures but are absent in LiCl treated cultures. DIC, digital interference contrast. (C) Wnt3a conditioned medium (Wnt3a) inhibited the formation of TRAP-positive multi-nucleated cells compared with cells incubated with control conditioned medium (L-Cell) over 12 days in culture. Values are mean ± s.e.m., n=5; ***P≤0.001; n.s., not significant.





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