spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Expression of LRP5 and LRP6 Wnt co-receptors by osteoblasts, osteoclast progenitors and mature osteoclasts. (A) RT-PCR demonstrating expression of LRP5 and LRP6 using cDNA generated from human osteoblastic cells (MG-63, SaOS-2 and TE85), primary human osteoblasts (HOB), mouse spleen and mature osteoclasts derived from human long-term bone marrow cultures. (B) Histogram illustrating the purity of CD14+ cells determined by flow cytometry following isolation from the mononuclear (MNC) fraction of human peripheral blood by magnetic activated cell sorting. RT-PCR demonstrating expression of LRP5 and LRP6 by CD14- (depleted) and CD14+ (enriched) cells is shown on the right. The PCR product obtained using SaOS-2 cDNA is shown for comparison.





Right arrow Return to article