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Figure 2


Fig. 2. Rabip4 and Glut 4 define distinct intracellular compartments. Endogenous Rabip4 and Glut 4 were studied by immunofluorescence. (a,b) Rabip4 was detected using rabbit antibodies to Rabip4 followed by FITC-coupled donkey anti-rabbit antibodies. (c, d) Glut 4 was detected using goat anti-Glut 4 antibodies followed by Texas-Red-coupled donkey anti-goat antibodies. (e-h) Merged images of Rabip4 and Glut 4 labeling prepared using Adobe Photoshop. Panels g and h are enlarged views of the boxes in panels e and f. Left and right columns correspond to confocal sections obtained near the bottom and in the middle of the adipocyte, respectively. Bar, 1 µm.





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