The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLC

JCS02835 Supplementary Material

Files in this Data Supplement:

• Movie 1 - Movie 1. TIRF analysis of an MCF10A cell spreading.  Time-lapse movie (1 image/second) of the still images shown in Figure 1B, in which a representative MCF10A cell is spreading on 10 mg/ml fibronectin for 40 minutes.

• Movie 2 - Movie 2. TIRF analysis of an MCF10A cell expressing R-Ras^38V during spreading.  Time-lapse movie (1 image/second) of the still images shown in Figure 1B, in which a representative MCF10A cell stably expressing R-Ras^38V is spreading on 10 mg/ml fibronectin for 40 minutes. 

• Movie 3 - Movie 3. Control MCF10A cells migrate on fibronectin. Control MCF10A cells were plated on Petri plates coated with 20 mg/ml fibronectin for 90 minutes and a time-lapse sequence (1 image/minute) acquired for 100 minutes.

• Movie 4 - Movie 4. R-Ras38V-expressing MCF10A cells cannot migrate despite the enhanced protrusive activity during cell spreading. Cells stably expressing activated R-Ras^38V  were plated on 20 mg/ml fibronectin for 90 minutes and a time-lapse sequence (1 image/minute) acquired for 100 minutes.