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Fig. 1. Ins(3,4,5,6)P₄ inhibits Ca²⁺-dependent Cl^- secretion across WT and CF MTE monolayers. (A-D) I_SC across WT (blue traces) and CF (red traces) MTE monolayers was measured as described in Materials and Methods. Incubation time was set to zero at the point at which UTP (100 µM) was added to the apical chamber. Where indicated, Bt₂Ins(3,4,5,6)P₄/PM and Bt₂Ins(1,4,5,6)P₄/PM were added to the apical chamber of the Ussing apparatus at a concentration of 50 µM, 2 hours prior to the addition of UTP. KN93 was added at a concentration of 5 µM, 2 hours prior to UTP addition. Representative traces are shown in A-D. (E) Graphs show the average height of I_SC peaks for the various experiments (mean ± s.e.m. of 4-6 determinations). Numbers above each bar indicate its percentage value relative to vehicle-treated cells within the same group [either WT (blue bars) or CF (red bars)].

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