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Figure 4


Fig. 4. Changes in levels of Ins(1,4,5)P3, Ins(1,3,4,5)P4, Ins(1,3,4)P3 and Ins(3,4,5,6)P4 following purinergic activation of MTEs. WT ({blacktriangleup}) and CF (bullet) MTEs were labeled for 4 days with [3H]inositol. Cells were treated with vehicle (the zero time point) or UTP (for 30, 60 or 90 seconds). Levels of the individual inositol phosphate are as indicated. (A), Ins(1,4,5)P3; (B), Ins(1,3,4,5)P4; (C), Ins(1,3,4)P3; (D), Ins(3,4,5,6)P4. Data represent mean ± s.e.m. of five experiments. *P<0.03; **P<0.02.





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