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Figure 7


Fig. 7. Analysis by confocal immunofluorescence microscopy of ITPK1 localization in polarized MTE monolayers. Cells were grown on membrane filters and prepared for immunofluorescence confocal microscopy as described in Materials and Methods. (A-G) Cells photographed in a horizontal (X-Y) optical section, with a corresponding vertical (X-Z) optical scans; (A-D) WT cells, (E-G) CF monolayers. Nuclei were stained with DAPI (A,E; blue), CFTR was stained with Alexa Fluor 546-labeled antibody (B; red) and ITPK1 was stained with Alexa Fluor 488-labeled antibody (C,F; green). The X-Z images of D and G include a white signal that is the confocal reflection from the filter. Bars, 10 µm.





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