QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. L1 localization to presynaptic terminals in the Schaffer-collateral pathway. (A) Overview of staining by anti-L1 antibody with DAB in the hippocampus of adult mice. The arrowheads indicate strongly stained black bundles from CA3 soma and immunoreactive fibers projected toward the stratum radiatum in the CA1 subfield (arrow). Stratum oriens (str. ori.), stratum pyramidale (str. pyr.), stratum radiatum (str. rad.) and lacunosum-moleculare (lac-mol.) are indicated. (B) Expression of L1 mRNA in hippocampus detected by in situ hybridization. Intense hybridization signals in the pyramidal layer, especially in CA3 pyramidal cells, appear as silver grains under dark-field illumination. (C) Higher magnification of L1ir axon bundles from CA3 soma marked by arrowheads in A. (D) Immunoelectron microscopic analysis of regions marked by arrowheads in A and C. L1ir is observed on and in axons (arrowheads). (E) Higher magnification of L1ir fibers marked by arrow in A. Some fasciculated axons show L1ir (arrows). (F) Higher magnification of an L1ir axon in the CA1 stratum radiatum. Arrowheads indicate L1ir boutons on axons. (G-I) Immunoelectron microscopic localization of L1 by pre-embedding staining in the CA1 stratum radiatum. L1ir is distributed in presynaptic sites of asymmetrical synapses (open arrowheads). L1ir was observed in orphan boutons containing a few vesicles (I). Closed arrowheads are L1ir-negative asymmetrical synapses. (J-L) Immunogold labeling of L1ir in CA1 stratum radiatum. Gold particles (10 nm, indicated by open arrowheads) are associated with the presynaptic membrane, the presynapse interior (J,K), and orphan boutons (L). Closed arrowheads indicate L1ir-negative asymmetrical synapses. Bars, 300 µm (A,B); 100 µm (C); 1 µm (D); 5 µm (E,F); 300 nm (I for G-I; J-L).

Return to article