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Fig. 4. Change in the distribution of L1ir sites in NP^-/- mice. (A) Low-power immunoelectron micrographs of the proximal region of the CA1 stratum radiatum in NP^+/+ (upper panel) and NP^-/- (lower panel) mice. There are more L1ir synaptic (arrowheads) and L1ir orphan boutons (asterisks) in NP^-/- mice than in NP^+/+ mice. Arrows show L1ir-negative synapses. Bar, 1 µm. (B) Western blot analysis of L1 in hippocampus of NP^+/+ and NP^-/- mice. Both the 80 and 200 kDa L1ir bands were detectable. Actin served as internal control for protein loading. The graph shows the quantification of band intensities of L1₂₀₀ and L1₈₀. The band density of L1 was significant increase in NP^-/- mice. ^*P<0.01.

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