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Fig. 8. Correlation of cell and fibril motion. (A) Vector maps depicting the direction of movement of point markers on cells and fibrils in day 2 FRC cell cultures. These vector maps were generated from still images from Movie 1 in supplementary material. 100 point markers were positioned on features in a still image of cells or fibrils. The same features were identified on an image obtained 1 hour later and the markers repositioned if they had moved. Vectors depicting the displacement of the point markers were generated in which the dots represent the initial position of each point marker, and the end of the line represents the position to which the point marker has moved. Note that the direction of movement of point markers on cell and fibril images appears generally correlated. (B) Graphs showing correlation of the direction of movement of vectors on cell and fibril images from day 2 cultures compared using the `local average' analysis (see Materials and Methods section). This software calculates the average angle of vector movement within paired local neighborhoods within a 30 µm radius from comparison images of fibronectin/LTBP1 or cells/fibronectin. The pairs of angles from equivalent neighborhoods in each image were plotted and the correlation coefficient calculated. Note that there was a strong correlation between the movement of fibronectin and LTBP1 vectors, as expected because these two ECM molecules were colocalized. The movement of vectors on cell images was also significantly correlated with the movement of fibronectin vectors. Similar results were obtained comparing cell and LTBP1 vectors (data not shown).
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