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Figure 1


Fig. 1. ßH-spectrin distribution in middle midgut cells. (A) Top: schematic diagram of the larval middle midgut. fg, foregut; pv, proventriculus; gc, gastric caece; am, anterior midgut; cc, cuprophilic cell region; mm, middle midgut; fe, iron cell region; pm, posterior midgut; bb, brush border (illustrates how the long microvilli of the brush border almost fill the apical invagination). Bottom: diagrams illustrating sagittal section (left) and cross-section (right) through the cuprophilic cell (CC) region. For orientation, a dashed box surrounds a cell colored to resemble the staining in C. (B-D) Wild-type third-instar CCs co-stained for {alpha}-spectrin (B, {alpha}-Sp, red) and ßH-spectrin (D, ßH-Sp, green) and merged (C). ßH and {alpha}-spectrin co-label the terminal web subtending the apical surface. {alpha} is also present on the basolateral membrane and is concentrated at the septate junctions (B, arrowheads). Insets show a top-down view of the septate junction region of another cell. *, CC nucleus. (E,F) karst mutant third-instar cells stained for {alpha}-spectrin ({alpha}-Sp). {alpha}-spectrin is not present at the terminal web in the absence of ßH. The CC in (E) has a properly positioned nucleus (*), whereas the cells in (F) have mispositioned nuclei. Other examples of nuclear mislocalization are seen in Fig. 3J-L and Fig. 6C. (G-I) Parasaggital section of a wild-type third-instar CC co-labeled with {alpha}-spectrin (G, {alpha}-Sp, green) and septate junction marker Coracle (I, Cor, red). The merged image (H) shows co-localization at the highly folded junction (bracket). Insets are a top-down view of another septate junction. (J-L) Saggital section of a wild-type third-instar CC co-labeled with ßH-spectrin (J, green) and Coracle (L, red). The apical and basolateral domain abut at the apical margin of the junctions (arrowheads in K). Insets show a top-down view of another septate junction. Bars, 20 µm, except the inset in B, which is 10 µm.





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