|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. V-ATPase staining of third-instar intestines. All samples were co-stained for 
-spectrin (A,D,G,J) and V-ATPase (C,F,I,L). Panels B, E, H and K are merged images (-spectrin in green, V-ATPase in red). (A-C) Wild-type gut. Cell shapes are revealed by -spectrin, which outlines both the apical and basolateral domains and exhibits high cytoplasmic concentrations in the interstitial cell cytoplasm. CCs in the center present in cross-section as rounded shapes with the nucleus in the center; by contrast, cells on the top and bottom edges of the gut are parasagittal with a cup-shaped apical invagination around the nucleus. The V-ATPase signal is visible at the apical domain and in clustered vesicular structures. (D-F) karst mutant gut. -spectrin stains only the basolateral membranes in the absence of ßH. V-ATPase staining is diffusely cytoplasmic, lacking both apical and vesicular concentrations. (G-I) Vertical cross-section of an individual wild-type CC. -spectrin stains both basolateral membrane and the terminal web (G, arrows) on either side of the apical invagination (G, asterisk). The terminal web in the center of the cell runs parallel to the image plane and dips into focus near the nucleus where again elevated V-ATPase can be seen (chevron in H). V-ATPase co-localizes with -spectrin in the terminal web at this resolution (H, arrow in I), and is also seen in numerous cytoplasmic vesicles near the basal membrane (H, arrowheads in I). (J-L) Vertical cross-section of an individual karst mutant CC. -spectrin stains the basolateral membrane only in this cell. The V-ATPase signal is diffuse and cytoplasmic, demonstrating neither an apical accumulation nor a vesicular concentration. Note also the misplaced nucleus (
in K). This view is in a similar plane to that illustrated in Fig. S1D (supplementary material). Bars, 20 µm.
|
