QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. V-ATPase is located in a subset of Rab5-positive endosomes in CCs. Cells shown are all from third-instar guts except where noted; `red' and `green' refer to colors in the central merged image. (A-C) Wild-type cell stained for V-ATPase (A, red) and the Golgi marker Lava Lamp (C, Lva, green). No co-localization is evident. (D-F) Wild-type cell stained for V-ATPase (D, red) and the recycling endosome marker Rab11 (F, green). No co-localization is evident. (G-I) Wild-type cell stained for V-ATPase (G, red) and the early endosome marker Rab5 (I, green), showing that all V-ATPase-positive structures co-label for Rab5. However, not all Rab5-positive endosomes co-label for V-ATPase. (J-M) A myc-2xFYVE construct was expressed in first-instar larval CCs to label phosphatidylinositol 3-phosphate [PtdIns(3)P]-positive endosomes. These were stained for Myc (K/2xFYVE, red), Rab5 (L, green) and V-ATPase (M, blue). Three classes of early endosomes are revealed: those labeled with Rab5 alone (e.g. arrows in L), those co-labeled with Rab5 and myc-2xFYVE (e.g. arrowhead in K and L), or those labeled with Rab5 and V-ATPase (bracket in L and M). Also, myc-2xFYVE stains the basolateral but not the apical membrane. (N-P) karst mutant cell stained for V-ATPase (N, red) and Rab5 (P, green). Not only is the V-ATPase dispersed but none of the Rab5-positive compartments are evident. (Q-S) karst mutant cell stained for Rab11 (Q, red) and Lava Lamp (S, Lva, green), showing that recycling endosomes and Golgi are intact and indistinguishable from wild type in the absence of ß_H-spectrin. Bars, 20 µm.

Return to article