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Figure 7


Fig. 7. Co-expression of Orc2 with Orc1 suppressed induction of apoptosis by Orc1. (A) CHO cells were transfected with the indicated amounts of pFCgOrc1 and pFCgOrc2, and cell lysates were subjected to immunoblotting at 24 hours post-transfection using anti-FLAG antibody. (B) CHO cells were co-transfected with 1.5 µg pCgOrc1 and 0.5 µg pFCgOrc2. At 24 hours post-transfection, cells were stained with both anti-CgOrc1 (Orc1) and anti-FLAG (F-Orc2) antibodies. Merged images revealed common areas of localization (yellow). DIC, differential interference contrast.





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