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Figure 2


Fig. 2. Prospore membrane proteins accumulate at the SPB in spo14 and sso1 mutants. Wildtype (HI54), sso1 {Delta}/sso1 {Delta} (HI58) and spo14 {Delta}/spo14 {Delta} (HI51) cells expressing the prospore membrane markers GFP-Spo14K1098H (from pRS424-GFP-SPO14K1098H) or Dtr1-GFP (from pRS424-DTR1-GFP) were sporulated and examined by fluorescence microscopy. DNA was visualized using DAPI and SPBs were visualized using an RFP fusion to Mpc54p. Bars, 1 {Delta}m.





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