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Figure 4


Fig. 4. Sorbitol density gradient fractionation of sporulating wildtype, spo14 {Delta} and sso1 {Delta} cells. (A) Immunoblots of fractionated extracts from wild-type (AN120), spo14 (HI6), and sso1 (HI3) cells expressing Dtr1-GFP. The 14,000 g supernatant (S14), pellet (P14), 100,000 g supernatant (S100), pellet (P100) and 20-60% sorbitol gradient fractions (1-22) were probed with anti-GFP to detect Dtr1p-GFP, anti-Gas1p and anti-Dpm1p, or anti-Vps10p (details in Materials and Methods). (B) Quantification of the pixel intensity of each band was performed using AlphaEase FC4.0 imager software. bullet, WT; {blacksquare}, spo14; {blacktriangleup}, sso1.





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