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Fig. 5. The membrane-binding domain of Spo20p does not localize to prospore membrane precursor vesicles in the spo14 mutant. Wild-type (HI54), sso1
/sso1
(HI58) and spo14
/spo14
(HI51) cells expressing GFP-Spo20p51-91 (from pRS426-G20) were sporulated and examined by fluorescence microscopy. DNA was visualized using DAPI and SPBs were visualized using an RFP fusion to Mpc54p. Bars, 1
m.