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Fig. 5. Effect of KGF treatment on
2ß1hi cells. (A) Bar chart summarising the effect of KGF on expression of differentiation markers PAP, CK18 and AR (measured by FACS) in
2ß1hi cells. (B) Triple immunofluorescence study of
2ß1hi cells treated with KGF. AR, TRITC/red; PSA, FITC/green; DAPI, blue. Colocalisation is indicated in yellow. (C) RT-PCR for KGFR mRNA expression in primary prostate cell sub-populations. *PEC, primary culture of epithelial cells following subtraction of
2ß1hi basal cells. ß-actin levels were detected as a control (lower gel). (D) Summary of FACS analysis of AR expression in
2ß1hi cells untreated or treated with combinations of KGF and the ß1-blocking antibody. Data are mean ± s.e.m. of three experiments.