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Figure 8


Fig. 8. Association of PP2A with K8 and K18 in HT29 cells after hyposmotic stress. HRT18 and HT29 cells were cultured in isosmotic (I) or hyposmotic (H) conditions (6 hours, 37°C), then solubilized with 1% NP40 in PBS (4°C). (A) The lysates were then analyzed by blotting using antibodies to K8, K18, PP1 or PP2A. (B) Alternatively, the NP40 lysates were used to immunoprecipitate K8 and K18 followed by blotting using the indicated antibody. Asterisk indicates the antibody heavy chain band.





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