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Figure 3


Fig. 3. OLIG2-induced upregulation of p27Kip1 mRNA and protein. U12-1 cells were cultured in the absence of doxycycline (Tet-off medium) for the indicated times to induce OLIG2 expression. The amount of p27Kip1 mRNA was then determined by quantitative RT-PCR analysis (A) and that of p27Kip1 protein was evaluated by immunoblot analysis (B). The abundance of p27Kip1 mRNA was normalized by that of GAPDH mRNA and expressed relative to the normalized value for time zero; data are means ± s.d. of values from three independent experiments. The immunoblot was also reprobed with antibodies to OLIG2 and to actin. *P<0.05, **P<0.01 (Student's t-test).





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