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Figure 2


Fig. 2. nELAV RBPs are expressed in neurospheres and neural stem/progenitor cells in vitro. (A,B) The nELAV antibody (green) was used in combination with anti-Msi1 antibody (red) to label adult mouse neurospheres showing a strong overlapping expression of the two RBPs in the whole neurosphere (C, merged). (D,E) Colocalization of nELAV (green) and Msi1 (red) RBPs is more evident in single stem/progenitor cells. Most of the neurosphere-derived precursors show the expression of the two antigens with a different cellular distribution, mainly nuclear for Msi1 (F, merged, arrow). The same field is represented in the inset at a reduced size where nuclei are evidenced by DAPI counterstaining (arrow). (G,H,I) The proliferative state of the cell culture is attested by nELAV (green) and Ki67 (red) positive labeling. These two proteins are coexpressed in certain precursors, whereas nELAV is selectively expressed in others (arrowheads). The nuclear localization of Ki67 antigen is clearly visible (asterisks). Bars, 100 µm (A-C); 25 µm (D-I).





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