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Fig. 4. Real-time PCR-based identification and quantification of mRNAs associated to nELAV-mRNP complexes in adult neurospheres. Endogenous mRNPs were immunoprecipitated by anti-nELAV, anti-His5 and no antibody and the collected mRNA species were quantified by real-time RT-PCR. The normalized fold difference is indicated with respect to the sample where no antibody was used (mean value ± s.e.m.; one-way ANOVA, n=3, ***P<0.001 for anti-nELAV antibody compared with both controls).