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Figure 2


Fig. 2. TCF4 slows down ß-catenin in the nucleus and decelerates its nucleo-cytoplasmic shuttling. (A) Live cell images of mYFP-ß-cateninS45A and co-expressed TCF4-mCFP in HEK293T cells. Bar, 10 µm. (B) Compartment bleach analysis of ß-catenin shuttling in the absence or presence of co-expressed TCF4-mCFP. (C) Nuclear point bleach analysis of mYFP-ß-catenin mobility in the absence or presence of TCF4-mCFP. TCF4-mYFP mobility was determined in a separate experiment. The cartoons in B and C designate compartment bleaches and point bleaches, respectively.





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