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Figure 3


Fig. 3. BCL9 slows down ß-catenin nucleo-cytoplasmic shuttling. (A) Live cell images of mYFP-ß-cateninS45A and co-transfected mCFP-BCL9v in the absence (left panels) or presence (right panels) of mRFP-Pygopus2. Bar, 10 µm. (B) Compartment bleach analysis of mYFP-ß-catenin shuttling in the absence or presence of mCFP-BCL9v and mRFP-Pygopus2. mYFP-BCL9v shuttling was analysed in a separate experiment. (C) Nuclear point bleach analysis of mYFP-ß-catenin in the absence or presence of mCFP-BCL9v and mRFP-Pygopus2. The mobilities of mYFP-BCL9v and mYFP-Pygopus were determined in separate experiments. The cartoons in B and C designate compartment bleaches and point bleaches, respectively.





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