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Figure 6


Fig. 6. APC and axin mediate ß-catenin cytoplasmic accumulation by a CRM-1 independent mechanism. (A) Immunofluorescence staining of endogenous ß-catenin (first two panels) and live cell images of HEK293T cells expressing mYFP-ß-cateninS45A or mYFP-ß-cateninNES in the presence of the indicated interactors (other panels) before and after 1 hour of leptomycin B treatment. Bars, 10 µm. (B) Compartment bleach analysis of mYFP-ß-catenin shuttling before and after 1 hour of leptomycin B treatment. The cartoon designates compartment bleaches.





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