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Fig. 7. Downregulation of B-Myb by RNA interference leads to chromosome instability. HEL cells treated with 10–8 M TPA for 16 hours were subjected to three rounds of transfection with 50 nM of the different siRNAs for 3 hours at intervals of one day. At 2 hours after the final treatment, chromosome spreads were prepared and stained using anti-CENPa (orange) and DAPI (green). Representative chromosome spreads are shown for cells treated with (A) control siRNA and (B) siRNA1 targeted against B-myb. The arrows in B indicate examples of aberrant chromosomes. (C) Expanded images of the types of chromosome aberration resulting from B-myb knockdown, including single chromatids (i,ii), chromosomes joined end-to-end (iii,iv), loss of chromosome segments (v,vi), and greater than two centromeres (vii,viii).
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