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Figure 2


Fig. 2. Arl8 targeting is evolutionarily conserved but brefeldin A resistant. (A) Confocal micrographs of COS cells expressing the Drosophila Arl8 homologue (CG7891) with GFP at the C-terminus (Dm Arl8-GFP), and after fixation and permeabilisation labelled with antibody against the lysosomal protein CD63. (B) Confocal micrographs of COS cells expressing Arl8b-GFP and either untreated, or incubated in 5 µg/ml brefeldin A for 15 minutes, before fixation and labelling with the indicated antibodies to residents of the lysosome (LAMP2) and the Golgi (giantin). Bars, 10 µm.





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