spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Arl8b expression results in a more peripheral localization of lysosomes. (A) Confocal micrographs of COS cells expressing Arl8b-GFP or Arl8b(Q75L)-GFP and labelled with the antibodies to the indicated lysosomal membrane proteins. (B) As in (A), except that cells were trypsinised and plated on glass slides 90 minutes prior to fixation. (C) As in (A), except that cells were treated with 20 µM nocodazole for 4 hours before fixation, and then labelled for tubulin to confirm microtubule depolymerisation, or for the lysosomal antigen CD63. In such cells the lysosomes are scattered throughout the cytosol irrespective of the presence of Arl8b-GFP. Bars, 10 µm.





Right arrow Return to article