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Figure 7


Fig. 7. Localisation of Arl8b in mitotic cells. Projection images of a stack of confocal slices from NRK cells transfected with a plasmid expressing Arl8b-GFP. After fixation and permeabilisation the cells were stained with antibodies to lysosomes and tubulin. Mitotic cells were identified in the total population by tubulin staining. Arl8b-GFP is localised to lysosomes, and is not detectable on the spindle mid-zone (A) or the midbody (B). Bars, 10 µm.





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