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Figure 10


Fig. 10. The complex of dynamin II and syndapin II promotes vesicle formation at Golgi-enriched membranes in vitro. The protein fraction 6 (obtained by fractionation of cytosolic proteins of HepG2 cells on a glycerol gradient) that contains complexes of dynamin II with syndapin II, as demonstrated by co-immunoprecipitation with anti-syndapin II antibodies (3685) and immunoblotting with anti-syndapin II and anti-dynamin II antibodies (A), is sufficient for promoting vesicle formation at Golgi-enriched membranes in vitro (B, circles). The stimulatory activity can be removed from fraction 6 by immunoprecipitation with anti-syndapin II antibodies (B, squares).





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