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Figure 3


Fig. 3. Co-localization of dynamin IIaa-GFP and Flag-syndapin II-l. (A-C) COS-7 cells cotransfected with dynamin IIaa-GFP and epitope-tagged syndapin II-l detected by GFP fluorescence (A) and anti-Flag immunostaining (C), respectively, show a complete spatial overlap of both proteins in the merged image (B; syndapin in red and dynamin in green; overlap appears yellow). Co-localization can be observed both at the perinuclear area and in the periphery of the cells. Insets represent twofold enlargements of boxed areas. Bar, 10 µm.





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