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Figure 4


Fig. 4. Dynamin II and syndapin II interact in vivo. (A) Complexes of dynamin II with syndapin II were immunoprecipitated from HepG2 cell extracts using anti-syndapin II antibodies (3685) and the anti-dynamin (Dyn) II antibody sc-6401, which recognizes an internal sequence of dynamin II, respectively. Eluates were analyzed by immunoblotting using the antibodies 3685 (anti-syndapin II) and C12 (anti-dynamin II). The antibody C12, which specifically recognizes the C-terminus of dynamin II, only immunoprecipitated dynamin II, but did not co-immunoprecipitate syndapin II. (B) The supernatants of the immunoprecipitations were precipitated with methanol and then immunoblotted with anti-dynamin II and anti-syndapin II antibodies. IPs, immunoprecipitates; CoIPs, coimmunoprecipitates.





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