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Figure 9


Fig. 9. Acute interference with syndapin functions by introducing anti-syndapin antibodies blocks Golgi-to-PM transport of VSVG-GFP. (A,B) VSVG-GFP accumulated in the Golgi at the start of chase (A) and reached the plasma membrane after 90 minutes (end of chase) in cells treated with BioPorter (BP) and anti-syndapin preimmune (B). (C,D) By contrast, cells treated with BioPorter and anti-syndapin immunoreagent showed an arrest of VSVG-GFP signal in the perinuclear area. Images were processed by Adobe Photoshop to visualize clearly the different VSVG-GFP distributions. Bars, 10 µm. (E) Quantitative analyses of fluorescence signals in the perinuclear regions of interest clearly show the undisturbed Golgi-to-PM trafficking in cells treated with BioPorter (BP alone) or BioPorter plus anti-syndapin preimmune (BP anti-Sdp preimmune), and the inhibition of Golgi exit of VSVG-GFP in cells into which anti-syndapin antibodies (BP anti-Sdp) had been introduced. Error bars represent standard deviations between independent data sets.





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