JCS02868 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Colocalization of p21-GFP and CPDs at the micro irradiated spot in a C2C12 myoblast. p21 transfected C2C12 cells were fixed 25 min after 405 nm laser microirradiation. CPDs were detected with monoclonal antibody. Arrow marks site of irradiation. p21-GFP accumulates at sites of DNA damage and co-localizes with CPDs. Bar, 5 mm.

• Supplemental Figure 2 -

  Fig. S2. Short term kinetics analysis of p21-GFP and RFP-PCNA fluorescence after 405 nm laser microirradiation in HeLa cells. Signals were acquired every 2 seconds and confocal sections are shown of images taken at the indicated times. The arrows indicate the site of irradiation. Bar, 5 mm.

• Supplemental Figure 3 -

  Fig. S3. Localisation of UV-induced CPDs and p21 protein, in locally irradiated XPA cells. After exposure to UV-C (15 J/m²) through filters with 3 mm-pores, cells were lysed in situ and fixed. Immunostaining with anti-CPDs monoclonal antibody (green fluorescence), and anti-p21 polyclonal antibody (red fluorescence) was performed after mild DNA denaturation with DNase I, to expose UV-lesions. Green spots indicate the presence of CPDs, while yellow spots indicate the presence of both UV-lesions and p21, at irradiated areas. Bar, 10 mm.