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Figure 5


Fig. 5. Colocalisation of p21-GFP with DNA repair proteins recruited to DNA-damage sites. HeLa cells expressing p21-GFP were locally irradiated with UV-C (10 J/m2) through 3 µm pores. After 30 minutes, cells were extracted in situ and fixed for determination of chromatin-bound p21-GFP and immunofluorescence staining of DNA repair proteins. (A) Confocal sections of p21-GFP (green) and pol {delta} (red) fluorescence signals are displayed together with the merged image also showing DNA counterstaining with Hoechst 33258 (blue). (B) confocal sections showing the single and merged images of p21-GFP (green), and XPG (red) at the irradiated sites. DNA was counterstained with Hoechst 33258 (blue). (C) Confocal sections showing the recruitment of p21-GFP (green) and CAF-1 (red), and DNA counterstaining (blue). Bars, 5 µm.





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