spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. p21 recruitment to DNA-damage sites depends on DNA repair activity. LF1 and XP20PV (XPA) fibroblasts were exposed to local UV-C irradiation (15 J/m2) through filters with 3 µm pores, extracted in situ, and fixed at the indicated times for determination of chromatin-bound PCNA and p21. Samples were immunostained with anti-PCNA polyclonal, and anti-p21 monoclonal antibody, detected respectively with secondary antibody conjugated with Alexa Fluor 488 (green fluorescence) or Alexa Fluor 594 (red fluorescence); DNA was counterstained with Hoechst 33258 (blue fluorescence). Bar, 10 µm.





Right arrow Return to article