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Figure 2


Fig. 2. {alpha}II spectrin colocalizes with G{alpha}q/11 at the periphery of M1-CHO cells. (A) Expression of the surface marker CD8 (red) is at the plasma membrane, whereas YFP-{alpha}II spectrin (green) is distributed in the cytosol with enrichment under the plasma membrane (merge). YFP-{alpha}II spectrin is not expressed in the second cell in this field because of its low transfection efficiency (maximum 30%) compared with CD8 (80-90%). (B) Endogenous G{alpha}q/11 (red) is distributed throughout the cytoplasm and colocalization with YFP-{alpha}II spectrin (green) is seen at the membrane skeleton (merge). (C) Myc-tagged G{alpha}q (red) similarly colocalizes with YFP-{alpha}II spectrin (green) at the plasma membrane skeleton (merge). Insets in merged images show higher magnification views of the boxed region of the main images. Bar, 10 µm.





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