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Figure 5


Fig. 5. Muscarinic agonist-induced spectrin remodeling occurs subsequent to a rise in [Ca2+]i. Activation of M1 mAChRs causes a transient increase in [Ca2+]i in M1-CHO cells. This rapid rise in [Ca2+]i (<20 seconds) precedes redistribution of YFP-{alpha}II spectrin, and spectrin redistribution (solid bar on the top indicates duration) continues after [Ca2+]i returned to the basal levels. The graph represents a typical response of Fura-2AM-loaded M1-CHO cells, and the average duration of spectrin redistribution (n=12).





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