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Fig. 7. Expression of nNOS is not restored by expression of Dp116. (A) Immunofluorescence staining of quadriceps muscles with a polyclonal antibody against nNOS. Dp116/mdx^4cv and transgenic/mdx muscles expressing Dp260, ${Delta}$ H2-R19 (mini-dystrophin), and ${Delta}$ R4-23 (micro-dystrophin) are shown. Bar, 100 µm. (B) Western analysis of nNOS from pellet (microsomal) and supernatant (soluble) fractions of KCl-washed microsome preparations from C57Bl/6 (1), mdx^4cv (2), Dp116/mdx^4cv (3), and ${Delta}$ R4-23/mdx (4) skeletal muscles. An identical gel stained in parallel with Coomassie Blue is shown as a loading control.

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