spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. S. cerevisiae twinfilin (Twf1) binds to and depolymerizes actin filaments in a pH-dependent manner. (A) Coomassie-stained SDS-polyacrylamide gel of purified Twf1. Positions of molecular mass markers (kDa) are indicated. (B,C) Time course of actin filament fluorescence (rabbit skeletal muscle actin; 30% pyrene labeled) after dilution at time zero (from 5 µM to 0.1 µM) alone or in the presence of Cof1, Twf1 or Lat-A at pH 5.0 (B) or pH 5.8 (C). The inset legend for B also applies to C. (D) Initial rate of actin filament fluorescence decrease induced by 250 nM Twf1 (reaction conditions as in B,C) as a function of pH in the reaction. The rate of fluorescence decrease for actin alone was set at 1 to reference Twf1 effects. (E,F) Time course of actin filament fluorescence (S. cerevisiae actin; 30% pyrene labeled) after dilution at time zero (from 5 µM to 0.1 µM) alone or in the presence of Cof1, Twf1 or Lat-A at pH 5.0 (E) or pH 7.5 (F).





Right arrow Return to article